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Phospho-RPS6 (Ser240 + Ser244) Rabbit pAb (bs-3389R)  
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產(chǎn)品編號 bs-3389R
英文名稱 Phospho-RPS6 (Ser240 + Ser244) Rabbit pAb
中文名稱 磷酸化S6核糖體蛋白(Ser240/244)抗體
別    名 RPS6(phospho S240+S244); p-RPS6(phospho S240+S244); Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal(1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S  
產(chǎn)品類型 磷酸化抗體 
研究領域 染色質和核信號  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat
產(chǎn)品應用 WB=1:100-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser240/244: RA(p-S)TSK(p-S)ES 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Function:
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

Post-translational modifications:
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Similarity:
Belongs to the ribosomal protein S6e family.

SWISS:
P62753

Gene ID:
6194

Database links:

Entrez Gene: 6194 Human

Entrez Gene: 20104 Mouse

Entrez Gene: 667739 Mouse

Entrez Gene: 29304 Rat

Omim: 180460 Human

SwissProt: P62753 Human

SwissProt: P62754 Mouse

SwissProt: P62755 Rat

Unigene: 408073 Human

Unigene: 379007 Mouse

Unigene: 425090 Mouse

Unigene: 34341 Rat



產(chǎn)品圖片
Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Phospho-RPS6 (Ser240 + Ser244) (bs-3389R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Phospho-RPS6 (Ser240 + Ser244) (bs-3389R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with(Phospho-RPS6(Ser240+Ser244)) Polyclonal Antibody, Unconjugated (bs-3389R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-RPS6 (Ser240+Ser244) Polyclonal Antibody, Unconjugated(bs-3389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-RPS6 (Ser240+Ser244) Polyclonal Antibody, Unconjugated(bs-3389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(black line):HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-RPS6 (Ser240 + Ser244) antibody (bs-3389R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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