| 產(chǎn)品編號(hào) | bs-0287R |
| 英文名稱 | His tag Rabbit pAb |
| 中文名稱 | His tag標(biāo)簽抗體 |
| 別 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
 |
Specific References (17) | bs-0287R has been referenced in 17 publications.
|
| 產(chǎn)品類型 | 標(biāo)簽抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 合成與降解 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Synthetic peptide: HHHHHH conjugated to KLH |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
標(biāo)簽抗體 蛋白質(zhì)純化的新途徑─應(yīng)用His-Tag抗體檢測His-Tag融合蛋白 1） 6xHis與不同的Sepharose純化填料結(jié)合（捆綁），可不受構(gòu)象的影響，在天然或變形的條件下可進(jìn)行進(jìn)一步純化。 |
| 產(chǎn)品圖片 |
20 ng rGFP-His protein (bs-33009P) per lane probed with His tag polyclonal antibody respectively, unconjugated (bs-0287R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
|
