| 產(chǎn)品編號 | bs-1251R |
| 英文名稱 | CD166 Rabbit pAb |
| 中文名稱 | 活化白細胞粘附分子抗體 |
| 別 名 | activated leukocyte cell adhesion molecule; ALCAM protein; ALCAM; CD 166; CD166 antigen; FLJ38514; MEMD; MGC71733; FLJ3851; AI853494; BEN; DM-GRASP; MGC27910; MuSC; SC1; CD166_HUMAN. |
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Specific References (10) | bs-1251R has been referenced in 10 publications.
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| 研究領域 | 腫瘤 細胞粘附分子 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD166: 451-583/583 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for CD166. The expression of CD166 is up-regulated in low-grade prostate tumors and down-regulated in high-grade tumors; may play role in progression of prostate cancer. Function: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and hemophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains. SWISS: Q13740 Gene ID: 214 Database links: Entrez Gene: 214 Human Entrez Gene: 11658 Mouse Omim: 601662 Human SwissProt: Q13740 Human SwissProt: Q61490 Mouse Unigene: 591293 Human Unigene: 288282 Mouse Unigene: 5789 Rat ALCAM/CD166是免疫球蛋白超家族的成員,對多種腫瘤生長和轉(zhuǎn)移相關的腫瘤細胞特性具有調(diào)節(jié)作用,ALCAM/CD166在乳腺癌、前列腺癌、食道癌、結腸癌及惡性黑色素瘤等腫瘤都有一定的表達，尤以結腸、直腸癌表達顯著。 |
| 產(chǎn)品圖片 |
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (bs-1251R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (bs-1251R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
Blank control: Molt-4 Cells(blue). Primary Antibody: Rabbit Anti-CD166/FITC Conjugated antibody (bs-1251R/AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1251R/AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
