亚洲制服欧美另类-午夜激情av电影-日本高清中文字幕一区二区三区-中国欧美日韩一区二区三区-欧洲亚洲日本韩国-成人欧美激情一区二区-亚洲偷偷自拍高清

掃碼關(guān)注公眾號           掃碼咨詢技術(shù)支持           掃碼咨詢技術(shù)服務(wù)
  
客服熱線:400-901-9800  客服QQ:4009019800  技術(shù)答疑  技術(shù)支持  質(zhì)量反饋  關(guān)于我們  聯(lián)系我們
国产精品va无码一区二区臀,国产精品 久久久精品软件下载,亚洲一级毛片无码专区
首頁 > 產(chǎn)品中心 > 一抗 > 產(chǎn)品信息
EpCAM Rabbit pAb (bs-1513R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術(shù)支持:techsupport@bioss.com.cn
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-1513R
英文名稱 EpCAM Rabbit pAb
中文名稱 上皮細胞粘附分子(CD326)抗體
別    名 Adenocarcinoma associated antigen; CD326; CD326 antigen; Cell surface glycoprotein Trop 1; CO17 1A; EGP; EGP40; Ep CAM; Epithelial cell surface antigen; Epithelial cellular adhesion molecule; Epithelial glycoprotein; GA733 2; hEGP 2; KS 1/4 antigen; KSA;   
Specific References  (8)     |     bs-1513R has been referenced in 8 publications.
[IF=6.799] Smriti Arora. et al. Antibody mediated cotton-archetypal substrate for enumeration of circulating tumor cells and chemotherapy outcome in 3D tumors. Lab Chip. 2022 Feb;:  Other ;  Other.  
[IF=5.396] Xiuxiu Wang. et al. Very-light alcohol consumption suppresses breast tumor progression in a mouse model. Food Funct. 2022 Mar;:  IF ;  Mouse.  
[IF=5.256] Jia M et al. A platform for primary tumor origin identification of circulating tumor cells via antibody cocktail-based in vivo capture and specific aptamer-based multicolor fluorescence imaging strategy. Analytica Chimica Acta.2019 July.  Other ;  Human.  
[IF=4.599] Shen, Xintong. et al. Evaluation of EpCAM-specific exosomal lncRNAs as potential diagnostic biomarkers for lung cancer using droplet digital PCR. 2021 Oct 14  WB ;  Human.  
[IF=4.08] Gerlach, J?rg C., et al. "Epithelial Cell Adhesion Molecule Fragments and Signaling in Primary Human Liver Cells." Journal of Cellular Physiology (2017).  IHC-P ;  Human.  
[IF=3.553] Liu M et al. Copper promotes sheep pancreatic duct organoid growth by ATOX1-dependent MEK-ERK pathway activation. Am J Physiol Cell Physiol. 2020 Apr 1;318(4):C806-C816.  IF ;  sheep.  
[IF=3.197] Jia M et al. Effect of dietary phytochemicals on the progression of breast cancer metastasis based on the in vivo detection of circulating tumor cells. Journal of Functional Foods 65 (2020) 103752.  In vivo capture of CTCs ;  Mouse.  
[IF=0] Ahn, Junsik, Kyung Jin Lee, and Kisung Ko. "Optimization of ELISA Conditions to Quantify Colorectal Cancer Antigen-Antibody Complex Protein (GA733-FcK) Expressed in Transgenic Plant." Monoclonal antibodies in immunodiagnosis and immunotherapy 33.1 (2014): 1-7.  ELISA ;  
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  細胞粘附分子  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 35 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human EpCAM: 221-314/314 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Function:
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Subunit:
Monomer. Interacts with phosphorylated CLDN7.

Subcellular Location:
Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Co-localizes with CLDN7 at the lateral cell membrane and tight junction.

Tissue Specificity:
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.

Post-translational modifications:
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

DISEASE:
Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.

Similarity:
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain.

SWISS:
P16422

Gene ID:
4072

Database links:

Entrez Gene: 4072 Human

Entrez Gene: 17075 Mouse

Entrez Gene: 171577 Rat

Omim: 185535 Human

SwissProt: P16422 Human

SwissProt: Q99JW5 Mouse

SwissProt: O55159 Rat

Unigene: 542050 Human

Unigene: 4259 Mouse

Unigene: 106481 Rat



產(chǎn)品圖片
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: K562 (Human) Cell Lysate at 30 ug Primary: Anti-EpCAM (bs-1513R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM ) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:K562. Primary Antibody (green line): Rabbit Anti-EpCAM antibody (bs-1513R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: FHC. Primary Antibody (green line): Rabbit Anti-EpCAM antibody (bs-1513R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版權(quán)所有 2004-2026 www.a6308.cn 北京博奧森生物技術(shù)有限公司
通過國際質(zhì)量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫(yī)療器械-質(zhì)量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網(wǎng)安備110107000727號
无码永久免费AV网站 | 一级做a爰片久久毛片免费陪 | 91亚洲精品无码久久久久 | 亚洲色婷婷久久99精品91 | 午夜福利麻豆国产精品 | 亚洲熟妇无码乱子AV电影 | 日韩欧美一区二区中文字幕 | 男女无遮挡XX00动态图120秒 | 欧美精品久久久久久久小说 | 亚洲第一成年人网站 | 黄色视频免费在线观看 | 国产午夜人做人免费视频APP | 国产成人AV在线播放影院 | 久久91亚洲精品中文字幕 | 美女国产毛片A区内射 | 亚洲一区二区免费 | 惩罚抽插校花嗯啊白浆 | 欧美日韩一区二区综合在线视频 | 又大又粗又爽的少妇免费视频 | 2012中文字幕免费高清 | 中国性少妇内射XXXX狠干 | 国产99精品在线观看 | 人人人人爽人人人人爱 | 中文字幕无码中文字幕有码 | 日产国产亚洲精品系列 | 国产在线a免费观看 | 国产在线观看www鲁啊鲁免费 | 青春草无码精品视频在线观看 | 精品人妻aV中文字幕乱 | 亚洲色欲色欲WWW成人网麻豆 | 99狠狠噜夜夜澡人人爽人人爽 | 91人妻无码精品一区二区毛片 | 一二三四五在线播放免费观看中文版 | 好紧好爽好疼的免费视频全集完整版在线播放 | 一级一级女人18毛片 | 国产99视频在线观看 | 把手戳进美女尿口里动态图 | 最近2019中文字幕免费直播 | 女人赤裸裸正面下身照片 | 九九视频免费精品视频免费 | 无码毛片内射白浆视频 |