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GlyR alpha 1 + 2 + 3 Rabbit pAb (bs-1524R)  
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50ul/1180.00元
100ul/1980.00元
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產(chǎn)品編號(hào) bs-1524R
英文名稱(chēng) GlyR alpha 1 + 2 + 3 Rabbit pAb
中文名稱(chēng) 甘氨酸受體alpha 1/2/3型抗體
別    名 glycine receptor alpha 1; glycine receptor alpha 2; glycine receptor alpha 3; GLRA1; GLRA2; Glycine receptor 48 kDa subunit; Glycine receptor alpha 2; Glycine receptor subunit alpha 3; Glycine receptor subunit alpha 2; Glycine receptor, alpha 1 subunit; G  
研究領(lǐng)域 免疫學(xué)  神經(jīng)生物學(xué)  通道蛋白  細(xì)胞膜受體  G蛋白偶聯(lián)受體  G蛋白信號(hào)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 49 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GlyR alpha 1: 101-200/449 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.

Subcellular Location:
Cell Membrane

SWISS:
Q6DJV9

Gene ID:
2742

Database links:

Entrez Gene: 2741 Human

Entrez Gene: 14654 Mouse

Entrez Gene: 25674 Rat

Entrez Gene: 281783 Cow

Entrez Gene: 489172 Dog

Omim: 138491 Human

SwissProt: P57695 Cow

SwissProt: P23415 Human

SwissProt: Q64018 Mouse

SwissProt: P07727 Rat

Unigene: 121490 Human

Unigene: 89320 Mouse

Unigene: 10109 Rat



甘氨酸受體alpha 1是中樞神經(jīng)系統(tǒng)中的重要抑制性離子通道受體,是哺乳類(lèi)中樞神經(jīng)系統(tǒng)中神經(jīng)遞質(zhì)受體蛋白,屬于配體門(mén)控離子通道超家族成員.
產(chǎn)品圖片
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Mouse Testis tissue lysates Lane 4: Mouse Lung tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Rat Cerebellum tissue lysates Lane 7: Rat Testis tissue lysates Lane 8: Human HeLa cell lysates Lane 9: Human A431 cell lysates Lane 10: Human SH-SY5Y cell lysates Primary: Anti-GlyR alpha 1 + 2 + 3 (bs-1524R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 53 kDa
Sample: Spinal cord (Mouse) Lysate at 40 ug Spinal cord (Rat) Lysate at 40 ug Primary: Anti-GlyR alpha 1 + 2 + 3 (bs-1524R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 54 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GlyR alpha 1/2/3 Polyclonal Antibody, Unconjugated(bs-1524R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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