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[KO驗(yàn)證抗體] CK18 Rabbit pAb (bs-1339R)  
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50ul/1180.00元
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200ul/2800.00元
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產(chǎn)品編號(hào) bs-1339R
英文名稱 [KO驗(yàn)證抗體] CK18 Rabbit pAb
中文名稱 細(xì)胞角蛋白18抗體(C端)
別    名 K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;  
Specific References  (7)     |     bs-1339R has been referenced in 7 publications.
[IF=6.208] Ting Lin. et al. CTH/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells. INT J MOL SCI. 2022 Jan;23(19):11822  IF ;  Mouse.  
[IF=4.292] Longfei Xiao. et al. Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. J Steroid Biochem. 2021 Nov;214:106001  IF ;  Bovine.  
[IF=4.147] Francesca Salamanna. et al. Development and characterization of a novel human 3D model of bone metastasis from breast carcinoma in vitro cultured. Bone. 2021 Feb;143:115773  IHC ;  Human.  
[IF=4.014] Xiaxia Du. et al. Circadian miR-218-5p targets gene CA2 to regulate uterine carbonic anhydrase activity during egg shell calcification. POULTRY SCIENCE. 2022 Aug;:102158  IF ;  Chicken.  
[IF=1.858] Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 Aug;:  IF ;  Sheep.  
[IF=1.56] Huang, Chao, et al. "Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis. Corrigendum in/10.3892/mmr. 2016.5882." Molecular Medicine Reports 14.3 (2016): 2555-2565.  IHC-P ;  Human.  
[IF=1.438] Jiang?JY et al. Effects of estradiol and progesterone on secretion of epidermal growth factor and insulin-like growth factor-1 in cultured yak endometrial epithelial cells. Tissue Cell. 2018 Jun;52:28-34.  ICC ;  Yak.  
研究領(lǐng)域 腫瘤  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Rabbit,Chicken,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2μg /test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 48 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CK18: 301-423/430 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Post-translational modifications:
Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P05783

Gene ID:
3875

Database links:

Entrez Gene: 506480 Cow

Entrez Gene: 3875 Human

Entrez Gene: 16668 Mouse

Entrez Gene: 294853 Rat

SwissProt: P05783 Human

SwissProt: P05784 Mouse

SwissProt: Q5BJY9 Rat



結(jié)構(gòu)蛋白(Structural Proteins)
常用于腫瘤細(xì)胞的分化、增殖及轉(zhuǎn)移方面的研究。
產(chǎn)品圖片
Sample: Hela KO CK18 (Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-CK18 (bs-1339R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: A549 (Human) Cell Lysate at 30 ug Lane 4: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-CK18 (bs-1339R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 45 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK18) Polyclonal Antibody, Unconjugated (bs-1339R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK18) Polyclonal Antibody, Unconjugated (bs-1339R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (CK18) polyclonal Antibody, Unconjugated (bs-1339R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): Hep G2 (blue). Primary Antibody (green line): Rabbit Anti-CK18 antibody (bs-1339R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Hela. Primary Antibody (green line): Rabbit Anti-CK18 antibody (bs-1339R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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