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Reprimo Rabbit pAb (bs-1885R)  
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產(chǎn)品編號 bs-1885R
英文名稱 Reprimo Rabbit pAb
中文名稱 細(xì)胞質(zhì)內(nèi)的糖基化蛋白抗體
別    名 Protein reprimo.; reprimo, TP53 dependent G2 arrest mediator candidate; 2410012A13RIK; FLJ90327; MGC109515; REPRIMO; RPRM; RPRM_HUMAN.  
Specific References  (1)     |     bs-1885R has been referenced in 1 publications.
[IF=1.28] Buchegger, Kurt, et al. "Immunohistochemical expression of RPRM is associated with low expression of proliferation marker Ki67 in patients with breast cancer." Int J Clin Exp Med 9.2 (2016): 3025-3032.  IHC-P ;  Human.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  細(xì)胞凋亡  細(xì)胞周期蛋白  糖蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Rat (predicted: Mouse,Rabbit,Cow)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 12 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Reprimo: 41-109/109 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Reprimo, also known as TP53 dependent G2 arrest mediator candidate or RPRM, is a 109 amino acid cytoplasmic protein belonging to the Reprimo family, which is involved in the regulation of p53-dependent G2 arrest of the cell cycle. Reprimo may initiate cell cycle arrest by inhibiting CDC2 and nuclear translocation of the CDC2 cyclin B1 complex. A highly glycosylated protein, Reprimo also plays a role in cell cycle surveillance and DNA repair. Hypermethylation of Reprimo can lead to its transcriptional repression, which may increase pathogenesis of some types of human cancers. Reprimo has been identified as a potential biomarker for early detection multiple cancers. The gene encoding Reprimo maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome.

Function:
May be involved in the regulation of p53-dependent G2 arrest of the cell cycle. Seems to induce cell cycle arrest by inhibiting CDK1 activity and nuclear translocation of the CDC2 cyclin B1 comple.

Subcellular Location:
Cytoplasm. Membrane; Single-pass membrane protein (Potential).

Similarity:
Belongs to the reprimo family.

Gene ID:
56475

Database links:
Entrez Gene: 56475 Human


Reprimo一種新發(fā)現(xiàn)的位于細(xì)胞質(zhì)內(nèi)的糖基化蛋白,在細(xì)胞質(zhì)中占有優(yōu)勢.
有學(xué)者認(rèn)為:Reprimo是胃癌早期檢測的潛在生物標(biāo)記物。
產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Reprimo) Polyclonal Antibody, Unconjugated (bs-1885R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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