亚洲制服欧美另类-午夜激情av电影-日本高清中文字幕一区二区三区-中国欧美日韩一区二区三区-欧洲亚洲日本韩国-成人欧美激情一区二区-亚洲偷偷自拍高清

掃碼關(guān)注公眾號(hào)           掃碼咨詢技術(shù)支持           掃碼咨詢技術(shù)服務(wù)
  
客服熱線:400-901-9800  客服QQ:4009019800  技術(shù)答疑  技術(shù)支持  質(zhì)量反饋  關(guān)于我們  聯(lián)系我們
japanhdv女同,色久综合网精品一区二区
首頁(yè) > 產(chǎn)品中心 > 一抗 > 產(chǎn)品信息
phospho-Smad2 (Ser465) Rabbit pAb (bs-2224R)  
訂購(gòu)熱線:400-901-9800
訂購(gòu)郵箱:sales@bioss.com.cn
訂購(gòu)QQ:  400-901-9800
技術(shù)支持:techsupport@bioss.com.cn
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) bs-2224R
英文名稱 phospho-Smad2 (Ser465) Rabbit pAb
中文名稱 磷酸化Smad2抗體
別    名 Smad2(phospho S465); phospho-Smad2(Ser465); p-Smad2(Ser465); phospho-Smad2(S465); Mothers against decapentaplegic homolog 2; SMAD 2; Mothers against DPP homolog 2; Smad2; hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN.  
Specific References  (9)     |     bs-2224R has been referenced in 9 publications.
[IF=3.69]   WB ;  rat.  
[IF=9.988] Hao Ni. et al. Long term toxicities following developmental exposure to perfluorooctanoic acid: Roles of peroxisome proliferation activated receptor alpha. ENVIRON POLLUT. 2023 Jan;317:120722  WB ;  Chicken.  
[IF=5.895] Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):10121–10133  WB ;  Pig, Mouse.  
[IF=4.784] Zheng Wu. et al. FOXD3 suppresses epithelial–mesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22  WB ;  human.  
[IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062.  IHC-P ;  Rat.  
[IF=3.647] Li L et al. Anti-fibrotic effect of melittin on TRIM47 expression in Human embryonic lung fibroblast through regulating TRIM47 pathway. Life Sci. 2020 Sep 1;256:117893.  WB ;  Human.  
[IF=3.342] Feng Wang. et al. Metformin reduces myogenic contracture and myofibrosis induced by rat knee joint immobilization via AMPK-mediated inhibition of TGF-β1/Smad signaling pathway. CONNECT TISSUE RES. 2022 Jun 20  WB ;  Rat.  
[IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).  WB ;  Human.  
[IF=2.742] Zhao, Xin. et al. NFK prevent postoperative abdominal adhesion through downregulating the TGF-β1 signaling pathway. MOL BIOL REP. 2022 Nov;:1-10  WB ;  Rat, Human.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 腫瘤  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse (predicted: Rat,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-5000,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 58 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser465: CS(p-S)MS 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Function:
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Subunit:
Momomer; the absence of TGF-beta. Heterodimer; in the presence of TGF-beta. Forms a heterodimer with co-SMAD, SMAD4, in the nucleus to form the transactivation complex SMAD2/SMAD4. Interacts with AIP1, HGS, PML and WWP1. Interacts with NEDD4L in response to TGF-beta. Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with ACVR1B, SMAD3 and TRIM33. Interacts (via the MH2 domain) with ZFYVE9; may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2-alpha subunit, CREB-binding protein (CBP), EP300 and SKI. Interacts with SNON; when phosphorylated at Ser-465/467. Interacts with SKOR1 and SKOR2. Interacts with PRDM16. Interacts (via MH2 domain) with LEMD3. Interacts with RBPMS. Interacts with WWP1. Interacts (dephosphorylated form, via the MH1 and MH2 domains) with RANBP3 (via its C-terminal R domain); the interaction results in the export of dephosphorylated SMAD3 out of the nucleus and termination ot the TGF-beta signaling. Interacts with PDPK1 (via PH domain).

Subcellular Location:
Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

Tissue Specificity:
Expressed at high levels in skeletal muscle, heart and placenta.

Post-translational modifications:
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.

Similarity:
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.

SWISS:
Q15796

Gene ID:
4087

Database links:

Entrez Gene: 4087 Human

Entrez Gene: 17126 Mouse

Entrez Gene: 29357 Rat

Omim: 601366 Human

SwissProt: Q15796 Human

SwissProt: Q62432 Mouse

SwissProt: O70436 Rat

Unigene: 12253 Human

Unigene: 705764 Human

Unigene: 391091 Mouse

Unigene: 2755 Rat



轉(zhuǎn)錄調(diào)節(jié)因子(Transcriptin Regulators)
Smads家族是最新發(fā)現(xiàn)的TGF-β信號(hào)轉(zhuǎn)導(dǎo)途徑中一個(gè)重要的新基因家族.是TGF-β特異的細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)分子,SMAD2/3屬于受體激活的SMADs.有學(xué)者認(rèn)為:Smad2、Smad3在某些腫瘤中發(fā)生突變,有可能是一種腫瘤抑制基因。
Smad2/3蛋白與Smad1、Smad5有高度同源性。
產(chǎn)品圖片
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: HT1080 (Human) Cell Lysate at 30 ug Lane 3: Jurkat (Human) Cell Lysate at 30 ug Lane 4: HL60 (Human) Cell Lysate at 30 ug Primary: Anti-phospho-Smad2 (Ser465) (bs-2224R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-phospho-Smad2 (Ser465) antibody (bs-2224R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-Smad2 (Ser465) antibody (bs-2224R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版權(quán)所有 2004-2026 www.a6308.cn 北京博奧森生物技術(shù)有限公司
通過國(guó)際質(zhì)量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號(hào): 00124Q34771R2M/1100
通過國(guó)際醫(yī)療器械-質(zhì)量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號(hào): CQC24QY10047R0M/1100
京ICP備05066980號(hào)-1         京公網(wǎng)安備110107000727號(hào)
国产精品久久久久9999小说 | 青草草在线视频永久免费 | 欧美精品在线观看 | 91天堂最新在线观看 | 黄色视频软件免费下载 | 国产精品成人久久久久无码 | 三根一起进出h黄蓉太疼了 国产在线观看www鲁啊鲁免费 | 亚洲免费在线播放 | 亚洲AV无码乱码国产麻豆穿越 | 91在线一区二区 | 国产99久久亚洲综合精品 | 免费看一级一级人妻片 | 亚洲中文字幕精品久久app | 国产国拍亚洲精品永久软件 | 狠狠的撞进去嗯啊h女强男视频 | 欧美在线成人免费观看网站 | 又黄又湿免费高清视频 | 日韩AV片无码一区二区三区不卡 | 91精品手机国产在线播放 | 亚洲日本欧美产综合在线 | 欧美日韩成人VA精品一区二区三区不卡 | 熟妇人妻久久中文字幕麻豆网 | 在线播放中文字幕 | 久久国产精品-久久精品 | 国产真人无遮挡作爱免费视频 | 一本视频精品中文字幕 | 国产91三级精选国产 | 欧美一级久久久久久久久大 | 亚洲AV无码专区一级婬片毛片 | 亚洲一区二区三区乱码在线欧洲 | 亚洲欧美日韩中文久久 | 久久久久成人精品亚洲国产av综合 | 国产刺激高潮免费不卡 | 一级a性色生活片久久毛片 日韩欧美一中文字慕2O22 | 免费永久在线观看黄网站 | 日韩精品视频美在线精品视频 | 亚洲国产精品高清免费在线观看 | 精品成a人亚洲精品无码 | 亚洲色偷偷综合亚洲AV伊人蜜桃 | 色欲日本人妻久久久久久综合 | 无码国产精品一区二区免费16 |