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WISP2 Rabbit pAb (bs-5100R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-5100R
英文名稱 WISP2 Rabbit pAb
中文名稱 Wnt1誘導(dǎo)信號通路蛋白2抗體
別    名 CTGFL; CCN family member 5; CCN5; Connective tissue growth factor like protein; Connective tissue growth factor related protein 58; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58; CT58; CTGF L; CTGFL; CTGF-L; WISP 2; WISP-2; Wisp2; WISP2_HUMAN; WNT1 inducible signaling pathway protein 2; WNT1-inducible-signaling pathway protein 2.  
Specific References  (2)     |     bs-5100R has been referenced in 2 publications.
[IF=5.782] Zolfaghari, Sima. et al. Tissue distribution and transcriptional regulation of CCN5 in the heart after myocardial infarction. 2021 Dec 01  IHC ;  Mouse.  
[IF=2.127] Sun S et al. CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells. Cell Biol Int. 2019 Dec 30.  WB ;  Human.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  發(fā)育生物學(xué)  干細(xì)胞  生長因子和激素  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 24 kDa
檢測分子量
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CTGFL: 171-250/2502 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 WISP2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. WISP2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

Function:
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.

Similarity:
Belongs to the CCN family.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.

SWISS:
O76076

Gene ID:
8839

Database links:

Entrez Gene: 8839 Human

Omim: 603399 Human

SwissProt: O76076 Human

Unigene: 592145 Human



產(chǎn)品圖片
Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-WISP2 (bs-5100R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD
Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-WISP2 (bs-5100R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WISP2) Polyclonal Antibody, Unconjugated (bs-5100R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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