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CLIC1 Rabbit pAb (bs-5112R)  
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產(chǎn)品編號(hào) bs-5112R
英文名稱(chēng) CLIC1 Rabbit pAb
中文名稱(chēng) 氯離子通道蛋白27抗體
別    名 hRNCC; Nuclear chloride ion channel 27; p64CLCP; Chloride channel ABP; Chloride intracellular channel 1; Chloride intracellular channel protein 1; CLIC 1; CLIC1; CLIC1_HUMAN; G6 antibody hRNCC; NCC 27; NCC27; Nuclear chloride ion channel 27; Nuclear chlor  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  通道蛋白  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Rabbit,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 26 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NCC27: 101-200/241 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]

Function:
Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.

Subunit:
Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion. Interacts with AKAP9.

Subcellular Location:
Nucleus. Nucleus membrane; Single-pass membrane protein (Probable). Cytoplasm. Cell membrane; Single-pass membrane protein (Probable).

Tissue Specificity:
Expression is prominent in heart, placenta, liver, kidney and pancreas.

Post-translational modifications:
Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.

Similarity:
Belongs to the chloride channel CLIC family.
Contains 1 GST C-terminal domain.

SWISS:
O00299

Gene ID:
1192

Database links:

Entrez Gene: 1192 Human

Entrez Gene: 515646 Cow

Entrez Gene: 114584 Mouse

Entrez Gene: 594847 Pig

Entrez Gene: 406864 Rat

Omim: 602872 Human

SwissProt: Q5E9B7 Cow

SwissProt: O00299 Human

SwissProt: Q9Z1Q5 Mouse

SwissProt: Q6MG61 Rat

Unigene: 414565 Human

Unigene: 29524 Mouse

Unigene: 203139 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLIC1) Polyclonal Antibody, Unconjugated (bs-5112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLIC1) Polyclonal Antibody, Unconjugated (bs-5112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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