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phospho-CDC27 (Thr244) Rabbit pAb (bs-5268R)  
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產(chǎn)品編號 bs-5268R
英文名稱 phospho-CDC27 (Thr244) Rabbit pAb
中文名稱 磷酸化后期促進復合蛋白3抗體
別    名 ANAPC3; Anaphase Promoting Complex 3; Anaphase promoting complex protein 3; Anaphase Promoting Complex Subunit 3; Anaphase-promoting complex subunit 3; APC 3; APC3; APC-3; Cdc 27; Cdc27; Cdc-27; CDC27 homolog; CDC27_HUMAN; CDC27Hs; Cell division cycle 27;  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 細胞生物  免疫學  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Rat (predicted: Human,Rabbit,Pig,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 92 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244: PD(p-T)VP 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [PATHWAY] Protein modification; protein ubiquitination.

Subunit:
The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI (By similarity). Interacts with MCPH1.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.

Similarity:
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats.

SWISS:
P30260

Gene ID:
996

Database links:

Entrez Gene: 996 Human

Entrez Gene: 540660 Cow

Entrez Gene: 490924 Dog

Entrez Gene: 217232 Mouse

Entrez Gene: 360643 Rat

Omim: 116946 Human

SwissProt: P30260 Human

SwissProt: A2A6Q5 Mouse

SwissProt: Q4V8A2 Rat

Unigene: 463295 Human

Unigene: 89845 Mouse

Unigene: 198335 Rat



產(chǎn)品圖片
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-GSK-3 Beta (Ser21) Polyclonal Antibody, Unconjugated(bs-5368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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