| 產(chǎn)品編號 | bs-6060R |
| 英文名稱 | NARC1/PCSK9 Rabbit pAb |
| 中文名稱 | 神經(jīng)細胞凋亡調(diào)節(jié)轉(zhuǎn)化蛋白1/前蛋白轉(zhuǎn)化酶枯草溶菌素9抗體 |
| 別 名 | FH3; HCHOLA3; Hypercholesterolemia autosomal dominant 3; NARC 1; NARC-1; NARC1; Neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; PC 9; PC9; PCSK 9; PCSK9; PCSK9_HUMAN; Proprotein convertase 9; Proprotein convertase PC9; Pr |
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Specific References (7) | bs-6060R has been referenced in 7 publications.
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| 研究領域 | 心血管 細胞生物 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 干細胞 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCSK9/NARC1: 231-330/692 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. Function: The precursor protein but not the mature protein may form multimers. Subunit: The precursor protein but not the mature protein may form multimers. Subcellular Location: Secreted. Tissue Specificity: Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. Post-translational modifications: The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. Similarity: Belongs to the peptidase S8 family. Contains 1 peptidase S8 domain. SWISS: Q8NBP7 Gene ID: 255738 Database links: Entrez Gene: 255738 Human Entrez Gene: 100102 Mouse Omim: 607786 Human SwissProt: Q8NBP7 Human SwissProt: Q80W65 Mouse Unigene: 18844 Human Unigene: 133268 Mouse Unigene: 19195 Rat 前蛋白轉(zhuǎn)化酶枯草溶菌素9(PCSK9)是近年來新發(fā)現(xiàn)的一種與調(diào)節(jié)脂質(zhì)代謝密切相關的基因,編碼前蛋白轉(zhuǎn)化酶NARC-1。Narc-1/pcsk9可通過調(diào)節(jié)細胞表面的低密度脂蛋白受體(LDLR)水平,參與脂質(zhì)代謝,從而在動脈粥樣硬化性疾病的發(fā)生、發(fā)展中發(fā)揮重要作用。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant human PCSK9 protein, C-His (HEK293)( bs-43105P)
Primary: Anti-NARC1/PCSK9 (bs-6060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 63 kDa
Sample: Lane 1:A431(Human) Cell Lysate at 30 ug Lane 2:Uterus (Mouse) Lysate at 40 ug Primary: Anti- NARC1/PCSK9 (bs-6060R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD
Sample:
Lane 1: HepG2(Human) Cell Lysate at 30 ug
Lane 2: SW480(Human) Cell Lysate at 30 ug
Primary: Anti- NARC1/PCSK9 (bs-6060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 70 kD
2664號盒-20230308-WDD_20.0x-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1/PCSK9) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NARC1/PCSK9) polyclonal Antibody, Unconjugated (bs-6060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-NARC1/PCSK9 antibody (bs-6060R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
