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TRPC3 Rabbit pAb (bs-6702R)  
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產(chǎn)品編號 bs-6702R
英文名稱 TRPC3 Rabbit pAb
中文名稱 色氨酸相關(guān)蛋白3抗體
別    名 Htrp 3; Htrp3; MGC124333; Mtrp3; Receptor activated cation channel TRP3v Short transient receptor potential channel 3; Transient receptor potential cation channel subfamily C member 3; Transient receptor potential channel 3; TRANSIENT RECEPTOR POTENTIAL D  
研究領域 心血管  神經(jīng)生物學  信號轉(zhuǎn)導  轉(zhuǎn)錄調(diào)節(jié)因子  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 92 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TRPC3: 201-300/836 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 TRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.

Function:
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.

Subunit:
Interacts with TRPC1, ITPR1, ITPR3, MX1 and RNF24. Interacts with JPH2; the interaction is involved in maintaining Ca(2+) homeostasis in skeletal muscle and is mediated by JPH2 'Ser-165' phosphorylation.

Subcellular Location:
Cell Membrane; multi-pass membrane protein

Tissue Specificity:
Expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis.

Similarity:
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC3 sub-subfamily.
Contains 4 ANK repeats.

SWISS:
Q13507

Gene ID:
7222

Database links:

Entrez Gene: 7222 Human

Entrez Gene: 22065 Mouse

Entrez Gene: 60395 Rat

Omim: 602345 Human

SwissProt: Q13507 Human

SwissProt: Q9QZC1 Mouse

SwissProt: Q9JMI9 Rat

Unigene: 150981 Human

Unigene: 74363 Mouse

Unigene: 45385 Rat



產(chǎn)品圖片
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRPC3 Polyclonal Antibody, Unconjugated(bs-6702R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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