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EB2 Rabbit pAb (bs-7837R)  
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產(chǎn)品編號 bs-7837R
英文名稱 EB2 Rabbit pAb
中文名稱 微管相關(guān)末端結(jié)合蛋白2抗體
別    名 APC binding protein EB2; APC-binding protein EB2; EB1; EB2; End binding protein 2; End-binding protein 2; H.sapiens mRNA for novel T cell activation protein; MAPRE2; MARE2_HUMAN; Microtubule associated protein RP/EB family member 2; Microtubule-associated  
研究領(lǐng)域 細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  細(xì)胞周期蛋白  細(xì)胞骨架  細(xì)胞外基質(zhì)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human End binding protein 2: 101-200/327 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012].

Function:
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.

Subunit:
Interacts with DCTN1. Binds to the C-terminal domain of APC. Binds monomeric and polymerized tubulin.

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes.

Similarity:
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain.

SWISS:
Q15555

Gene ID:
10982

Database links:
UniProtKB/Swiss-Prot: Q15555

產(chǎn)品圖片
Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- EB2 (bs-7837R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EB2) Polyclonal Antibody, Unconjugated (bs-7837R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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