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ALAS1 Rabbit pAb (bs-9527R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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產(chǎn)品編號(hào) bs-9527R
英文名稱 ALAS1 Rabbit pAb
中文名稱 5-氨基乙酰丙酸合酶1抗體
別    名 mitochondrial; nonspecific; 5 aminolevulinate synthase; 5 aminolevulinate synthase nonspecific mitochondrial; 5 aminolevulinic acid synthase; 5-aminolevulinate synthase; 5-aminolevulinic acid synthase 1; Alas 1; ALAS 3; ALAS; ALAS H; ALAS N; ALAS-H; alaS1; ALAS3; ALASH; Aminolevulinate delta synthase 1; Aminolevulinic acid synthase 1; delta ALA synthetase; delta aminolevulinate synthase; delta-ALA synthase 1; delta-aminolevulinate synthase 1; HEM1_HUMAN; MIG 4; MIG4; Migration inducing protein 4.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 65 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ALAS1/ALAS-H: 161-260/640 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

Subunit:
Homodimer.

Subcellular Location:
Mitochondrion matrix

Similarity:
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

SWISS:
P13196

Gene ID:
211

Database links:

Entrez Gene: 211 Human

Entrez Gene: 11655 Mouse

Entrez Gene: 65155 Rat

Omim: 125290 Human

SwissProt: P13196 Human

SwissProt: Q8VC19 Mouse

SwissProt: P13195 Rat

Unigene: 476308 Human

Unigene: 290578 Mouse

Unigene: 97126 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALAS1) Polyclonal Antibody, Unconjugated (bs-9527R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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