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KLHL36 Rabbit pAb (bs-9628R)  
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產(chǎn)品編號(hào) bs-9628R
英文名稱 KLHL36 Rabbit pAb
中文名稱 Kelch樣蛋白36抗體
別    名 Kelch like 36; Kelch like protein 36; kelch-like 36(Drosophila); Kelch-like protein 36; KLH36_HUMAN; KLHL 36; KLHL-36.  
Specific References  (1)     |     bs-9628R has been referenced in 1 publications.
[IF=8.886] Hengzhi Du. et al. MiR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF. Mol Ther-Nucl Acids. 2021 Aug;:  IF ;  Rat, Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 70 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KLHL36/C16orf44: 51-150/616 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 C16orf44 is a 616 amino acid protein that contains six Kelch repeats, one BTB/POZ domain and one BTB/Kelch associated (BACK) domain. C16orf44 is believed to play a role in protein ubiquitination and may function as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, C16orf44 interacts with CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Due to alternative splicing events, two isoforms of C16orf44 are expressed.

Function:
Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Subunit:
Interacts with CUL3.

Similarity:
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.

SWISS:
Q8N4N3

Gene ID:
79786

Database links:

Entrez Gene: 79786 Human

Entrez Gene: 234796 Mouse

Entrez Gene: 498957 Rat

SwissProt: Q8N4N3 Human

SwissProt: Q8R124 Mouse

SwissProt: Q66HD2 Rat

Unigene: 578546 Human

Unigene: 86446 Mouse

Unigene: 139361 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human lung cancer ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLHL) Polyclonal Antibody, Unconjugated (bs-9628R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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