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CD168 Rabbit pAb (bs-4736R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-4736R
英文名稱 CD168 Rabbit pAb
中文名稱 透明質(zhì)酸介導(dǎo)細胞游走受體抗體
別    名 CD168 antigen; HMMR; HMMR_HUMAN; Hyaluronan mediated motility receptor; Hyaluronan-mediated motility receptor(RHAMM); IHABP; Intracellular hyaluronic acid-binding protein; Intracellular hyaluronic acid-binding protein; MGC119494; MGC119495; Receptor for hyaluronan-mediated motility; RHAMM; Hyaluronan mediated motility receptor.  
研究領(lǐng)域 細胞生物  免疫學(xué)  干細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse (predicted: Rat,Cow,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 84 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 細胞外基質(zhì) 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD168/RHAMM: 51-150/725 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan that regulates cell adhesion and migration. HA effects are mediated through two receptors, CD44 (also designated HCAM) and the receptor of hyaluronic acid mediated motility (RHAMM). RHAMM, also designated intracellular hyaluronic acid binding protein (IHABP) and CD168, is a matrix receptor, which is linked to the plasma membrane by a GPI anchor and regulates cell motility. RHAMM expression is upregulated in malignant lymphoid tissues and is subsequently implicated in tumor progression and metastasis formation, as well as signal transduction. Although still unclear, RHAMM is thought to exist as several isoforms ranging in size. A variant isoform, designated v4, is a protein that when over-expressed, is thought to be the cause of transformation and metastasis formation in fibroblasts.

Function:
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.

Subcellular Location:
Cell surface. Cytoplasm.

Tissue Specificity:
Expressed in breast cancer cell lines and in normal breast tissue.

SWISS:
O75330

Gene ID:
3161

Database links:

Entrez Gene: 3161 Human

Omim: 600936 Human

SwissProt: O75330 Human

Unigene: 728200 Human

Unigene: 740467 Human



產(chǎn)品圖片
Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-CD168 (bs-4736R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD168) Polyclonal Antibody, Unconjugated (bs-4736R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control(blue):MCF 7 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti- CD168/AF647 Conjugated antibody (bs-4736R-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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