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NPFF1 Receptor Rabbit pAb (bs-12018R)  
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50ul/1180.00元
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產(chǎn)品編號(hào) bs-12018R
英文名稱(chēng) NPFF1 Receptor Rabbit pAb
中文名稱(chēng) G蛋白偶聯(lián)受體147抗體
別    名 NPFF1 Receptor; G Protein Coupled Receptor 147; G-protein coupled receptor 147; GPR147; Neuropeptide FF receptor 1; NPFF1; NPFF1_HUMAN; NPFF1R1; NPFFR1; RFamide related peptide receptor OT7T022; RFamide-related peptide receptor OT7T022.  
Specific References  (2)     |     bs-12018R has been referenced in 2 publications.
[IF=3.333] Zixuan Chen. et al. Exogenous Melatonin Regulates Puberty and the Hypothalamic GnRH-GnIH System in Female Mice. BRAIN SCI. 2022 Nov;12(11):1550  WB ;  Mouse.  
[IF=2.751] Xueying Zhao. et al. Effects of RFRP?3 on an ovariectomized estrogen?primed rat model and HEC?1A human endometrial carcinoma cells. EXP THER MED. 2023 Feb;25(2):1-14  WB ;  Human.  
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞膜受體  G蛋白偶聯(lián)受體  G蛋白信號(hào)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 48 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GPR147/NPFF1 Receptor: 151-260/430 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Neuropeptide FF 1 Receptor (NPFF1 or hFF1) and Neuropeptide FF 2 Receptor (NPFF2) belong to the G protein-coupled receptor 1 family. Both NPFF1 and NPFF2 are integral membrane proteins that act as receptors for NPAF (A-18- F-amide) and NPFF (F-8-F-amide) neuropeptides. Both NPFF proteins may be activated by synthetic or naturally occurring FMRF-amide-like ligands. The receptors are mediated by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. NPFF1 Receptors is highly expressed in the human hypothalamus and amygdala, indicating a possible role for NPFF1 in central autonomic and neuroendocrine control in the human brain. Based in part on NPFF2 Receptor expression in diencephalon and superficial layers of the spinal cord, NPFF2 Receptor is thought to be involved in the modulation of sensory input and opioid analgesia.

Function:
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular Location:
Cell membrane.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
Q9GZQ6

Gene ID:
64106

Database links:

Entrez Gene: 64106 Human

Entrez Gene: 64107 Rat

Omim: 607448 Human

SwissProt: Q9GZQ6 Human

SwissProt: Q9EP86 Rat

Unigene: 302026 Human

Unigene: 64483 Rat




產(chǎn)品圖片
Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-NPFF1 Receptor (bs-12018R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Sample: Lane 1: Mouse Cerebellum tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Cerebellum tissue lysates Lane 4: Rat Cerebrum tissue lysates Primary: Anti-NPFF1 Receptor (bs-12018R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (bs-12018R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (bs-12018R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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