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CLIC6 Rabbit pAb (bs-12038R)  
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產(chǎn)品編號(hào) bs-12038R
英文名稱 CLIC6 Rabbit pAb
中文名稱 氯離子通道蛋白6抗體
別    名 Chloride channel form A; Chloride intracellular channel 6; Chloride intracellular channel protein 6; CLIC1L; Clic6; CLIC6_HUMAN; Parchorin.  
研究領(lǐng)域 腫瘤  神經(jīng)生物學(xué)  通道蛋白  細(xì)胞膜受體  G蛋白偶聯(lián)受體  新陳代謝  G蛋白信號(hào)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 73 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CLIC6: 531-630/704 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CLIC6 (chloride intracellular channel 6) is believed to play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport. The CLIC6 gene is a rare example of large-scale segmental paralogy in which a large (approximately 500 kb) segment on human chromosome (HC) 21 (21q22) is triplicated on HC 1 and HC 6. CLIC6 is also known to interact with dopamine receptors DRD2, DRD3 and DRD4. CLIC6 is primarily expressed in the cytoplasm, however, upon chloride ion efflux from the cell, CLIC6 is translocated to the plasma membrane. CLIC6 has been identified in brain, placenta, pancreas and liver.

Function:
May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport.

Subunit:
Interacts with dopamine receptors DRD2, DRD3 and DRD4

Subcellular Location:
Cytoplasm. Cell membrane. Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane.

Tissue Specificity:
Expressed in brain, placenta, pancreas and liver.

Post-translational modifications:
Phosphorylated.

Similarity:
Belongs to the chloride channel CLIC family.
Contains 1 GST C-terminal domain.

SWISS:
Q96NY7

Gene ID:
54102

Database links:

Entrez Gene: 54102 Human

Omim: 615321 Human

SwissProt: Q96NY7 Human

Unigene: 473695 Human



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human neurilemmoma); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLIC6) Polyclonal Antibody, Unconjugated (bs-12038R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.
Blank control:Mouse kidney. Primary Antibody (green line): Rabbit Anti-CLIC6 antibody (bs-12038R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse kidney. Primary Antibody (green line): Rabbit Anti-CLIC6 antibody (bs-12038R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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