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Ribonuclease 3/ECP Rabbit pAb (bs-8615R)  
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產(chǎn)品編號 bs-8615R
英文名稱 Ribonuclease 3/ECP Rabbit pAb
中文名稱 嗜酸性粒細(xì)胞陽離子蛋白抗體
別    名 eosinophil cationic protein; EAR; Ear11; Ear4; EAR5; ECP; LOC192264; MGC143624; mR3; mR5; R1; R14; R15; R17; R5; R7; Ribonuclease 3; Rnase17; Rnase2; RNASE3; RNS3; ECP_HUMAN.  
Specific References  (4)     |     bs-8615R has been referenced in 4 publications.
[IF=12.05] Fujisawa, Daisuke, et al. "Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria." Journal of Allergy and Clinical Immunology (2014).  IHC-F ;  Human.  
[IF=4.082] Yoshimasa Imoto. et al. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. Biomolecules. 2020 Nov;10(11):1568  IF ;  Human.  
[IF=2.36] Inada, Noriko, et al. "Histamine H 1 and H 4 receptor expression on the ocular surface of patients with chronic allergic conjunctival diseases." Allergology International (2017).  IF(ICC) ;  Human.  
[IF=2.36] Tokunaga, Takahiro, et al. "The significant expression of TRPV3 in nasal polyps of eosinophilic chronic rhinosinusitis." Allergology International (2017).  IHC-P ;  Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  通道蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,ICC/IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 16 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Ribonuclease 3: 45-160/160 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 ECP is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis, and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases

Function:
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.

Subcellular Location:
Cytoplasmic granule. Matrix of eosinophil's large specific granule.

Similarity:
Belongs to the pancreatic ribonuclease family.

SWISS:
P12724

Gene ID:
6037

Database links:

Entrez Gene: 6037 Human

Entrez Gene: 53876 Mouse

Entrez Gene: 100361866 Rat

Entrez Gene: 192264 Rat

Omim: 131398 Human

SwissProt: P12724 Human

SwissProt: O35290 Mouse

SwissProt: P70709 Rat

Unigene: 73839 Human

Unigene: 327386 Mouse

Unigene: 10626 Rat



產(chǎn)品圖片
Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term)(Cat. #bs-8615R). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
RNASE3 Antibody (C-term) (Cat. #bs-8615R) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).
Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#bs-8615R) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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