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COLGALT1 Rabbit pAb (bs-13378R)  
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產(chǎn)品編號 bs-13378R
英文名稱 COLGALT1 Rabbit pAb
中文名稱 糖基轉(zhuǎn)移酶25結(jié)構(gòu)域1/前膠原半乳糖基轉(zhuǎn)移酶1抗體
別    名 GLT25D1; FLJ22329; Glycosyltransferase 25 domain containing 1; Hydroxylysine galactosyltransferase 1; Procollagen galactosyltransferase 1; GT251_HUMAN.  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導(dǎo)  新陳代謝  細胞外基質(zhì)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,ICC/IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 69 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLT25D1: 531-622/622 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 GLT25D1 is a 622 amino acid protein that localizes to the lumen of the endoplasmic reticulum. Ubiquitously expressed with higher levels in placenta, heart, lung and spleen, GLT25D1 is a member of the glycosyltransferase 25 family. The beta-galactosyltransferase activity of GLT25D1 allows the transfer of beta-galactose to hydroxylysine residues of collagen. GLT25D1 is considered an important target for investigating the biological significance of collagen glycosylation and the importance of posttranslational modification in the etiology of connective tissue disorders. The gene encoding GLT25D1 is located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.

Function:
Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen.

Subcellular Location:
Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin.

Tissue Specificity:
Ubiquitous with higher levels in placenta, heart, lung and spleen.

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the glycosyltransferase 25 family.

SWISS:
Q8NBJ5

Gene ID:
79709

Database links:

Entrez Gene: 513167 Cow

Entrez Gene: 484834 Dog

Entrez Gene: 100070236 Horse

Entrez Gene: 79709 Human

Entrez Gene: 234407 Mouse

Entrez Gene: 290637 Rat

SwissProt: A5PK45 Cow

SwissProt: Q8NBJ5 Human



產(chǎn)品圖片
Sample: Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Liver tissue lysates Lane 3: Rat Placenta tissue lysates Lane 4: Rat Spleen tissue lysates Lane 5: Rat Liver tissue lysates Lane 6: Human THP-1 cell lysates Primary: Anti-COLGALT1 (bs-13378R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 68 kDa
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