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GNL2 Rabbit pAb (bs-13471R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-13471R
英文名稱 GNL2 Rabbit pAb
中文名稱 鳥嘌呤核苷酸結(jié)合蛋白2抗體
別    名 Autoantigen NGP-1; Autoantigen NGP1; DJ423B22.6(novel nucleolar guanosine 5'-triphosphate binding protein; FLJ40906; GNL2; Guanine nucleotide binding protein-like 2(nucleolar); HUMAUANTIG; NGP1; NOG2_HUMAN; Nucleolar GTP binding protein 2; Nucleolar GTP-b  
研究領(lǐng)域 細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  G蛋白偶聯(lián)受體  表觀遺傳學(xué)  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 84 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GNL2: 1-100/731 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 GNL2 is a nucleolar guanasine-triphosphate binding protein that is ubiquitously expressed at low levels in almost all tissues. GNL2 is involved in the crucial process of trafficking proteins out of the nucleus. Specifically, it is a GTPase that interacts with the 60s preribosomal subunit in the nucleus and facilitates export of the subunit into the cytoplasm. GTPases are responsible for the hydrolysis of GTP by way of a protein region dubbed the G domain. GTPases are often involved in the translocating proteins through membranes gleaning energy for the activity by hydrolizing GTP. GNL2 shares G domain homology and some functionality with nucleostemin (GNL3), another nuclear GTPase. Highest expression of GNL2 is found in testis.

Function:
GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.

Subcellular Location:
Nucleus; nucleolus.

Tissue Specificity:
Ubiquitously expressed at relatively low levels in all human tissues tested, with the highest level of expression in the testes.

Similarity:
Belongs to the MMR1/HSR1 GTP-binding protein family. NOG2 subfamily.
Contains 1 G (guanine nucleotide-binding) domain.

SWISS:
Q13823

Gene ID:
29889

Database links:

Entrez Gene: 29889 Human

Entrez Gene: 230737 Mouse

Entrez Gene: 362593 Rat

Omim: 609365 Human

SwissProt: Q13823 Human

SwissProt: Q99LH1 Mouse

Unigene: 75528 Human

Unigene: 90760 Mouse

Unigene: 18964 Rat



產(chǎn)品圖片
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- GNL2 (bs-13471R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(bs-13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(bs-13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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