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hnRNP H2 Rabbit pAb (bs-17344R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-17344R
英文名稱 hnRNP H2 Rabbit pAb
中文名稱 異質(zhì)核核糖核蛋白H2抗體
別    名 FTP 3; FTP-3; FTP3; Heterogeneous nuclear ribonucleoprotein H2; HNRH2_HUMAN; hnRNP H2; HNRNPH2; HNRPH2.  
研究領域 細胞生物  結合蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse (predicted: Human,Rat,Pig)
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 49 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human hnRNP H2: 101-200/449 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. Read-through transcription between this locus and the ribosomal protein L36a gene has been observed. [provided by RefSeq, Jan 2011]

Function:
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG).

Subcellular Location:
Nucleus > nucleoplasm.

Tissue Specificity:
Expressed ubiquitously.

Similarity:
Contains 3 RRM (RNA recognition motif) domains.

SWISS:
P55795

Gene ID:
3188

Database links:

Entrez Gene: 3188 Human

Entrez Gene: 56258 Mouse

Entrez Gene: 308650 Rat

Omim: 300610 Human

SwissProt: P55795 Human

SwissProt: P70333 Mouse

SwissProt: Q6AY09 Rat

Unigene: 432485 Human

Unigene: 315909 Mouse

Unigene: 160642 Rat



產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with hnRNP H2 polyclonal antibody, unconjugated (bs-17344R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP H2) Polyclonal Antibody, Unconjugated (bs-17344R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP H2) Polyclonal Antibody, Unconjugated (bs-17344R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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