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ORC2 Rabbit pAb (bs-18022R)  
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產(chǎn)品編號 bs-18022R
英文名稱 ORC2 Rabbit pAb
中文名稱 嗅覺受體C2抗體
別    名 orc2; ORC2_HUMAN; ORC2L; Origin of replication 2(yeast homolog) like; Origin recognition complex protein 2 homolog; Origin recognition complex subunit 2; Origin recognition complex subunit 2 isoform B; Origin recognition complex subunit 2 like(yeast); Origin recognition complex, subunit 2 homolog; Origin recognition complex, subunit 2, S. cerevisiae, homolog of; Origin recognition complex, subunit 2-like(S. cerevisiae).  
研究領域 細胞生物  染色質(zhì)和核信號  信號轉(zhuǎn)導  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Chicken,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 66 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ORC2: 201-300/577 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]

Function:
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the ORC2 family.

SWISS:
Q13416

Gene ID:
4999

Database links:

Entrez Gene: 4999 Human

Entrez Gene: 18393 Mouse

Entrez Gene: 301430 Rat

Omim: 601182 Human

SwissProt: A6QNM3 Cow

SwissProt: Q13416 Human

SwissProt: Q60862 Mouse

SwissProt: Q75PQ8 Rat

Unigene: 444870 Human

Unigene: 3411 Mouse

Unigene: 75605 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ORC2) Polyclonal Antibody, Unconjugated (bs-18022R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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