| 產(chǎn)品編號 | bs-10762R |
| 英文名稱 | Secretogranin V Rabbit pAb |
| 中文名稱 | 神經(jīng)內(nèi)分泌顆粒蛋白5抗體 |
| 別 名 | 7B2; 7B2 protein; APPG; Neuroendocrine protein 7B2; P7B2; Pituitary polypeptide; Pituitary polypeptide; Prohormone convertase chaperone; SCG 5; SCG5; Secretogranin 5; Secretogranin V 7B2 protein; Secretogranin5; SecretograninV; Secretory granule endocrine |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17/21 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Secretogranin V: 1-100/212 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sg V is a 212 amino acid protein that is secreted by endocrine and neuroendocrine secretory granules and belongs to the 7B2 family. Existing as two alternatively spliced isoforms, Sg V interacts with PC2 and, via this interaction, functions as a molecular chaperone for PC2, effectively preventing its premature activation in regulated secretory pathways. More specifically, Sg V binds to PC2 and facilitates its transport from the endoplasmic reticulum to secretory compartments, thus allowing PC2 to be cleaved and activated during the correct phase of the regulated secretory pathway. Sg V is subject to post-translational sulfation on specific tyrosine residues and is underexpressed in medulloblastomas, suggesting a role in tumor suppression. The gene encoding Sg V maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Function: Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a C-terminal peptide. It is the C-terminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable. Subunit: Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages. Subcellular Location: Secreted. Note=Neuroendocrine and endocrine secretory granules. Tissue Specificity: Highly expressed in brain. Expressed at much lower level in other tissues. Post-translational modifications: Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides. Sulfated on tyrosine residues. Similarity: Belongs to the 7B2 family. SWISS: P05408 Gene ID: 6447 Database links: Entrez Gene: 6447 Human Entrez Gene: 20394 Mouse Omim: 173120 Human SwissProt: P05408 Human SwissProt: P12961 Mouse Unigene: 156540 Human Unigene: 4836 Mouse Unigene: 6173 Rat
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| 產(chǎn)品圖片 |
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Secretogranin V(bs-10762R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/21 kD
Observed band size: 21 kD
Protein: spleen(mouse) lysate at 40ug;
Primary: rabbit Anti-Secretogranin V (bs-10762R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:17/21 kD
Observed band size:21 kD
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Secretogranin V) Polyclonal Antibody, Unconjugated (bs-10762)t 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
