| 產(chǎn)品編號 | bs-23048R |
| 英文名稱 | CAPN1 Rabbit pAb |
| 中文名稱 | 鈣蛋白酶1抗體 |
| 別 名 | Calcium activated neutral proteinase 1; Calpain1; Calpain-1; Calpain 1; Calpain 1 catalytic subunit; Calpain large polypeptide L1; Calpain mu type; CANP 1; CANP; CANP1; CANPL1; CAPN 1; Cell proliferation inducing gene 30 protein; Cell proliferation induci |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Calpain 1: 521-620/713 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Subunit: Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Subcellular Location: Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. Tissue Specificity: Ubiquitous. Post-translational modifications: Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. Similarity: Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains. Database links: Entrez Gene: 12333 Mouse Entrez Gene: 823 Human Omim: 114220 Human SwissProt: P07384 Human SwissProt: O35350 Mouse Unigene: 502842 Human Unigene: 6221 Mouse Unigene: 6037 Rat 鈣蛋白酶(calpain)是一族鈣離子依賴性的、水解半胱氨酸的蛋白酶,廣泛存在于全身各臟器組織中。鈣蛋白酶參與不同細(xì)胞過程,包括細(xì)胞骨架和細(xì)胞膜附著的構(gòu)型重建、不同的信號轉(zhuǎn)導(dǎo)途徑和細(xì)胞凋亡等. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAN1) Polyclonal Antibody, Unconjugated (bs-23048R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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