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Transferrin Mouse mAb, Serum Loading Control (bsm-33243M)  
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50ul/680.00元
100ul/980.00元
500ul/4000.00元
200ug(PBS only)/5600.00元
大包裝/詢價(jià)

產(chǎn)品編號 bsm-33243M
英文名稱 Transferrin Mouse mAb, Serum Loading Control
中文名稱 轉(zhuǎn)鐵蛋白(內(nèi)參)單克隆抗體
別    名 Apotransferrin; TRF; eta 1 metal binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin precursor; Siderophilin; TF; TRFE_HUMAN; beta-1 metal-binding globulin; Serotransferrin.  
產(chǎn)品類型 內(nèi)參抗體 
研究領(lǐng)域 心血管  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 12A7
交叉反應(yīng) Human,Mouse,Rat,Cow
產(chǎn)品應(yīng)用 WB=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 77 kDa
檢測分子量
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Full length native Transferrin protein purified from human plasma 
亞    型 IgG
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009].

Function:
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Subunit:
Monomer.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed by the liver and secreted in plasma.

DISEASE:
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.

Similarity:
Belongs to the transferrin family.
Contains 2 transferrin-like domains.

SWISS:
P02787

Gene ID:
7018

Database links:

Entrez Gene: 7018 Human

Entrez Gene: 22041 Mouse

Entrez Gene: 24825 Rat

Omim: 190000 Human

SwissProt: P02787 Human

SwissProt: Q921I1 Mouse

SwissProt: P12346 Rat

Unigene: 518267 Human

Unigene: 37214 Mouse

Unigene: 91296 Rat



產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Transferrin monoclonal antibody, unconjugated (bsm-33243M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (bsm-33243M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (bsm-33243M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Blank control(black line):HepG2. Primary Antibody (green line): Mosue Anti-Transferrin(Serum Loading Control) antibody (bsm-33243M) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-mouse IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Mouse IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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