| 產(chǎn)品編號(hào) | bs-22005R |
| 英文名稱 | DOK1 Rabbit pAb |
| 中文名稱 | D酪氨酸激酶衰減蛋白1抗體 |
| 別 名 | docking protein 1(downstream of tyrosine kinase 1); Docking protein 1(p62(dok))(Downstream of tyrosine kinase 1)(pp62).; docking protein 1 62kD(downstream of tyrosine kinase 1); Docking protein 1 62kD; Docking protein 1 62kD; Docking protein 1; DOK 1; DOK |
| 研究領(lǐng)域 | 腫瘤 心血管 信號(hào)轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DOK1: 1-100/481 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. Subunit: Interacts with ABL1. Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3. Subcellular Location: Isoform 1: Cytoplasm. Isoform 3: Cytoplasm, perinuclear region. Tissue Specificity: Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Post-translational modifications: Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated by LYN. Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Isoform 3 contains a N-acetylmethionine at position 1. Similarity: Belongs to the DOK family. Type A subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: Q99704 Gene ID: 1796 Database links: Entrez Gene: 1796 Human Entrez Gene: 13448 Mouse Omim: 602919 Human SwissProt: Q99704 Human SwissProt: P97465 Mouse Unigene: 103854 Human Unigene: 156 Mouse Unigene: 117974 Rat 信號(hào)傳導(dǎo)（Signaling Intermediates） |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOK1) Polyclonal Antibody, Unconjugated (bs-22005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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