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FosB Recombinant Rabbit mAb (bsm-52071R)  
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50ul/1400.00元
100ul/2500.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) bsm-52071R
英文名稱 FosB Recombinant Rabbit mAb
中文名稱 FosB重組兔單抗
別    名 FBJ murine osteosarcoma viral oncogene homolog B; FOS B; FOS-B; FBJ osteosarcoma oncogene B; fosB; G0/G1 switch regulatory protein 3; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOS 3; GOS B; GOSB; MGC42291; Oncogene FOS B; Oncogene FOSB; Protein fos B; Protein fosB; DKFZp686C0818; FOSB_HUMAN.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號(hào)  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) 9C11
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 A synthesized peptide derived from human FOSB: 1-150/338 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, Fos B, (fos-related antigen) Fra 1, and Fra 2. Also named as DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB.

Function:
FosB interacts with Jun proteins enhancing their DNA binding activity.

Subunit:
Heterodimer.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.

SWISS:
P53539

Gene ID:
2354

Database links:

Entrez Gene: 2354 Human

Entrez Gene: 100360880 Rat

Omim: 164772 Human

SwissProt: P53539 Human

Unigene: 590958 Human



產(chǎn)品圖片
NIH/3T3 (M) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h,C6 (R) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with FosB monoclonal antibody, unconjugated (bsm-52071R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell (TPA stimulated); Triton X-100 at r.t. for 20 min; Antibody incubation with (FosB) monoclonal Antibody, unconjugated (bsm-52071R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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