| 產(chǎn)品編號 | bsm-51520M |
| 英文名稱 | PSMA5 Mouse mAb |
| 中文名稱 | 蛋白酶體PSMα5單克隆抗體 |
| 別 名 | Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome(prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome subu |
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Specific References (1) | bsm-51520M has been referenced in 1 publications.
[IF=4.081] Liu Jiayi. et al. Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells. BIOL TRACE ELEM RES. 2023 Apr;:1-14 WB ; Bovine.
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| 研究領(lǐng)域 | 細胞生物 細胞周期蛋白 細胞分化 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | N9G6 |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow) |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:10-50,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26.5 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PSMA5 (Fragment) (His-tagged). |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Subunit: The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in fetal brain (at protein level). Similarity: Belongs to the peptidase T1A family. SWISS: P28066 Gene ID: 5686 Database links: Entrez Gene: 5686 Human SwissProt: P28066 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: K562 cell lysates
Primary: Anti-PSMA5 (bsm-51520M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26.5 kD
Observed band size: 26 kD
Sample:
Lane 1: Human liver tissue lysates
Lane 2: Hela cell lysates
Lane 3: HepG2 cell lysates
Lane 4: MCF-7 cell lysates
Lane 5: Neuro-2a cell lysates
Lane 6: PC-12 cell lysates
Primary: Anti-PSMA5 (bsm-51520M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26.5 kD
Observed band size: 26 kD
Paraformaldehyde-fixed, paraffin embedded ( human testis section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMA5) Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PSMA5) monoclonal Antibody, Unconjugated (bsm-51520M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor? 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin .
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
