| 產品編號 | bsm-51716M |
| 英文名稱 | RNF4 Mouse mAb |
| 中文名稱 | RNF4單克隆抗體 |
| 別 名 | RNF4_HUMAN; E3 ubiquitin ligase RNF4; E3 ubiquitin protein ligase RNF4; Protein SNURF; RES4 26; RING finger protein 4; SLX5; Small nuclear ring finger protein; SNURF. |
| 研究領域 | 染色質和核信號 表觀遺傳學 泛素 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | E4R2 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human RNF4. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010] Function: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation. Subcellular Location: Cytoplasm. Nucleus. Nucleus. PML body. Nucleus. nucleoplasm. Tissue Specificity: Widely expressed at low levels in many tissues; highly expressed in testis. Post-translational modifications: Sumoylated; conjugated by one or two SUMO1 moieties. Autoubiquitinated. Similarity: Contains 1 RING-type zinc finger. SWISS: P61020 Gene ID: 5869 Database links: Entrez Gene: 51573 Human Omim: 605943 Human SwissProt: Q9NZC3 Human Unigene: 512607 Human |
| 產品圖片 |
Sample:
Lane 1: LNCap cell lysates
Lane 2: PC-3 cell lysates
Lane 3: Human testis tissue lysates
Primary: Anti-RNF4 (bsm-51716M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 34 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
