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ZCWCC1 Rabbit pAb (bs-25176R)  
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產(chǎn)品編號 bs-25176R
英文名稱 ZCWCC1 Rabbit pAb
中文名稱 ZCWCC1抗體
別    名 AC004542.C22.1.; CW type with coiled coil domain 1; KIAA0852; ZCW3; ZCWCC1; Zinc finger; Zinc finger CW type coiled coil domain protein 1; Zinc finger CW type with coiled coil domain 1; Zing finger CW type 3 zinc finger CW-type coiled-coil domain protein 1; MORC family CW-type zine finger 2; MORC2_HUMAN; MORC2.  
研究領(lǐng)域 染色質(zhì)和核信號  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 118 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ZCWCC1: 791-890/1032 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The CW domain is a structural module found in many vertebrate, parasitic and plant proteins. It consists of a mononuclear four-cysteine zinc-finger domain that plays a role in DNA binding, chromatin methylation and early embryonic development. ZCWCC1 (zinc finger CW-type coiled-coil domain protein 1), also known as MORC2 (MORC family CW-type zinc finger protein 2) or ZCW3, is a 1,032 amino acid protein that contains one CW-type zinc finger domain. ZCWCC1 is located on chromosome 22 and is ubiquitously expressed with highest expression in pancreas, smooth muscle and testis. Expression of ZCWCC1 is upregulated in hypoxia, a pathological condition characterized by an inadequate supply of oxygen in the blood.

Function:
May act as a transcriptional repressor. Down-regulates CA9 expression.

Subunit:
Interacts with HDAC4.

Subcellular Location:
Nucleus. Cytoplasm, cytosol. Note=Mainly located in the nucleus.

Tissue Specificity:
Highly expressed in smooth muscle, pancreas and testis.

Similarity:
Contains 1 CW-type zinc finger.

SWISS:
Q9Y6X9

Gene ID:
22880

Database links:

Entrez Gene: 22880 Human

SwissProt: Q9Y6X9 Human

Unigene: 143840 Human



產(chǎn)品圖片
Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-ZCWCC1 (bs-25176R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kDa Observed band size: 120 kDa
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ZCWCC1) Polyclonal Antibody, Unconjugated (bs-25176R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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