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FCGR1A/CD64 Rabbit pAb (bs-25232R)  
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產(chǎn)品編號(hào) bs-25232R
英文名稱 FCGR1A/CD64 Rabbit pAb
中文名稱 免疫球蛋白G Fc段受體I
別    名 High affinity immunoglobulin gamma Fc receptor I; CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; Fc fragment of IgG, high affinity Ia, receptor(CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high   
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  細(xì)胞膜受體  細(xì)胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse FCGR1A/CD64 : 131-230/404 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Subunit:
Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.

Tissue Specificity:
Monocyte/macrophage specific.

Similarity:
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Database links:

Entrez Gene: 2209 Human

Omim: 146760 Human

SwissProt: P12314 Human

Unigene: 77424 Human



CD64是一種糖蛋白,主要定位于單核細(xì)胞和巨噬細(xì)胞,參與免疫應(yīng)答反應(yīng)。
產(chǎn)品圖片
Sample: Lane 1: Mouse Lung tissue lysates Primary: Anti-FCGR1A/CD64 (bs-25232R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 62 kDa
Blank control(black line):Raw264.7. Primary Antibody (green line): Rabbit Anti-CD64 antibody (bs-25232R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat Anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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