| 產(chǎn)品編號 | bsm-60113R |
| 英文名稱 | Histone H3 (Tri Methyl K36) Recombinant Rabbit mAb |
| 中文名稱 | 三甲基化組蛋白H3抗體 |
| 別 名 | H3K36me3; Tri-Methyl-Histone H3(Lys36); Histone H3(Tri Methyl Lys36); Tri methyl Histone H3(Lys36); Tri methyl Histone H3(K36); H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; |
| 產(chǎn)品類型 | 甲基化抗體 重組兔單抗 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | B3C9 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Tri Methyl K36 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. SWISS: Q71DI3 Gene ID: 8968 組蛋白翻譯后修飾（PTMs）是表觀遺傳學調(diào)控染色質(zhì)結(jié)構(gòu)的關(guān)鍵機制，被稱為“組蛋白密碼”。組蛋白上的翻譯后修飾包括乙酰化，甲基化，磷酸化和近年發(fā)現(xiàn)的一些新型?；揎棥＿@些組蛋白修飾直接影響染色質(zhì)和轉(zhuǎn)錄因子或其他表觀調(diào)控子的結(jié)合，改變基因組的穩(wěn)定性和基因轉(zhuǎn)錄等。組蛋白甲基化通常發(fā)生在核心組蛋白的賴氨酸和精氨酸殘基。組蛋白甲基化既可以促進也可以抑制基因轉(zhuǎn)錄，這取決于組蛋白甲基化是發(fā)生在賴氨酸還是發(fā)生在精氨酸上以及甲基化基團的個數(shù)（賴氨酸可以發(fā)生一、二、三甲基化，精氨酸可發(fā)生單、對稱與非對稱二甲化）。組蛋白賴氨酸甲基化通常發(fā)生在組蛋白H3第4，9，27，36，79和組蛋白H4的第20位賴氨酸上；精氨酸甲基化通常發(fā)生在組蛋白H3的第2，8，17，26和組蛋白H4第3位精氨酸上。蛋白甲基化酶（HMT）和組蛋白去甲基化酶（HDM）是甲基化修飾主要的調(diào)控因子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1. MCF-7, 2. NIH-3T3, 3. HepG2, 4. Rat kidney, 5. Mouse kidney, 6. Recombinant Histone H3
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60113R
Tissue: Mouse stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60113R
Tissue: Rat kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60113R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
