| 產(chǎn)品編號 | bsm-60469M |
| 英文名稱 | STRAP Recombinant Mouse mAb |
| 中文名稱 | 絲氨酸/蘇氨酸激酶受體相關(guān)蛋白單克隆抗體 |
| 別 名 | MAP activator with WD repeats; MAWD; PTWD; serine/threonine kinase receptor associated protein; STRAP; UNR-interacting protein; UNRIP; WD 40 repeat protein PT WD; STRAP_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | F5B7 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,ICC/IF=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STRAP |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enables RNA binding activity. Involved in maintenance of gastrointestinal epithelium; negative regulation of transforming growth factor beta receptor signaling pathway; and spliceosomal snRNP assembly. Located in cytosol. Part of SMN complex. Implicated in adenocarcinoma; colorectal carcinoma; large cell carcinoma; lung carcinoma; and squamous cell neoplasm. [provided by Alliance of Genome Resources, Apr 2022] SWISS: Q9Y3F4 Gene ID: 11171 SMN復(fù)合物在小核核糖核蛋白（snRNPs）的組裝中起催化作用，snRNPs是剪接體的組成部分。因此，在細胞前mRNAs的拼接中起著重要的作用。大多數(shù)剪接體snRNP包含一組共同的Sm蛋白質(zhì)SNRPB、SNRPD1、SNRPD2、SNRPD3、SNRPE、SNRPF和SNRPG，它們在小核RNA的Sm位點上組裝成七聚體蛋白環(huán)，形成核心snRNP。在細胞質(zhì)中，Sm蛋白SNRPD1、SNRPD2、SNRPE、SNRPF和SNRPG被控制核心snRNP組裝的伴侶CLNS1A捕獲在非活性的6S pICln Sm復(fù)合物中。CLNS1A的SMN復(fù)合物從捕獲的Sm蛋白中解離并將其轉(zhuǎn)移到SMN-Sm復(fù)合物觸發(fā)核心snRNPs的組裝并將其轉(zhuǎn)運到細胞核。條帶在SMN復(fù)合體的細胞分布中起作用。負調(diào)節(jié)TGF-β信號，但通過增強其自身磷酸化和顯著減少PDPK1與14-3-3蛋白的結(jié)合來正調(diào)節(jié)PDPK1激酶活性。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 38 kDa
Observed MW: 38 kDa
Tissue: Human-lung-squamous-carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60469M
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60469M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60469M
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
