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Cdc42 Recombinant Rabbit mAb (bsm-60650R)  
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25ul/800.00元
50ul/1400.00元
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產品編號 bsm-60650R
英文名稱 Cdc42 Recombinant Rabbit mAb
中文名稱 細胞分裂周期蛋白42重組兔單抗
別    名 CDC 42; CDC42Hs; Cell division control protein 42 homolog; Cell division cycle 42; Cell division cycle 42 isoform 1; Cell division cycle 42 isoform 2; dJ224A6.1.1; dJ224A6.1.2; G25K; G25K GTP binding protein; Growth regulating protein; GTP binding protein  
研究領域 信號轉導  干細胞  轉錄調節(jié)因子  G蛋白偶聯(lián)受體  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 R1H7
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:50-1:100,IF=1:50,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 25 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cdc42 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody.

SWISS:
P60953

Gene ID:
998

Database links:

Entrez Gene: 998 Human

Entrez Gene: 12540 Mouse

Entrez Gene: 64465 Rat

Omim: 116952 Human

SwissProt: P60953 Human

SwissProt: P60766 Mouse

SwissProt: Q8CFN2 Rat



產品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: PC-3, 2: Mouse brain Protein loading quantity: 20 ug Exposure time: 60 s Predicted MW: 21 kDa Observed MW: 21 kDa
Tissue: Mouse colon Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60650R
Tissue: Rat colon Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60650R
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