| 產(chǎn)品編號 | IHC0111R | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 英文名稱 | Rat Histone H3 Ready-To-Use IHC Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 中文名稱 | 大鼠組蛋白H3（核內(nèi)參）單克隆即用型免疫組化試劑盒 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 別 名 | H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 種 屬 | Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 應用范圍 | Immunohistochemistry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 適用樣品基質(zhì) | FFPE tissue | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 描述 | IHC0111 is a ready-to-use IHC kit for staining of Histone H3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 產(chǎn)品介紹 |
產(chǎn)品組分及規(guī)格
背景 Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
石蠟包埋組織的免疫組織化學方案 1. 脫蠟水化 石蠟切片置于新鮮二甲苯中浸泡脫蠟3次，每次15 min；依次置于不同濃度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸餾水洗滌5 min，重復3次。 2. 抗原修復 沸水浴修復：將100×抗原修復緩沖液（試劑2）用蒸餾水稀釋成1×抗原修復緩沖液，放入修復盒中并提前加熱至95-100℃（注意蓋好以防液體蒸發(fā)），然后將切片放入修復盒中，在沸水浴環(huán)境中保持外沸狀態(tài)15 min，室溫自然冷卻；用PBS緩沖液（試劑1，將干粉溶解在2L蒸餾水中）清洗5 min，重復3次。 3. 阻斷內(nèi)源性過氧化物酶 用吸水紙吸去玻片上多余的液體，用免疫組化筆在組織周圍畫圈，加入2-4滴內(nèi)源性過氧化物酶阻斷劑（試劑3），室溫下置于濕盒中孵育15 min，用PBS洗滌5 min，重復3次。 4. 血清封閉 用吸水紙吸去玻片上多余的液體，加入2-4滴封閉工作液（試劑4），置于濕盒內(nèi)37℃封閉20 min，以減少非特異性染色。 5. 一抗孵育 用吸水紙吸去玻片上多余的液體，加入2-4滴大鼠Histone H3 鼠單抗工作液（試劑5），置于濕盒中，4℃孵育過夜或37℃孵育1-2 h。 6. 復溫 4℃孵育過夜后，室溫下復溫15 min (若在室溫下孵育一抗，則直接進入下一步清洗)；用PBS洗滌5 min，重復3次。 7. 二抗孵育 用吸水紙吸玻片上多余的液體，加入2-4滴HRP標記羊抗鼠IgG工作液（試劑6），置于濕盒中，37℃孵育1-2 h；用PBS洗滌5 min，重復3次。 8. 顯色 用吸水紙吸去玻片上多余的液體，在每張切片上滴加約50 μL新配制的DAB工作液（試劑7:試劑8:PBS=1:1:18），作用3-5 min。顯微鏡下觀察結(jié)果，達到合適的顯色強度后，用蒸餾水沖洗切片以終止反應，用蒸餾水沖洗5 min，重復3次。 9. 復染 滴加適量復染試劑（試劑9）復染3-5 min，蒸餾水沖洗5 min，滴加鹽酸酒精分化約30 s，蒸餾水洗滌5 min，重復2次。 10. 脫水封片 將玻片依次置于不同濃度（70%、80%、90%、95%、100%）乙醇，各5 min；然后置于新鮮二甲苯中浸泡脫蠟3次，每次15 min。用吸水紙吸去多余的二甲苯，滴加適量封片劑（試劑10）在組織上，將蓋玻片蓋在組織上，避免產(chǎn)生氣泡。
注意事項 1. 建議檢測時進行陰性及陽性對照，以提高實驗的可靠性。 2. 本品中的配套試劑，請不要用其他生產(chǎn)商產(chǎn)品替換使用。 3. DAB為致癌物質(zhì)，請采取必要的防范措施。 4. PBS洗滌液（試劑1）配制后在4℃可保存一周；抗原修復液（試劑2）及顯色劑（試劑7和8）的工作液需每次實驗時現(xiàn)用現(xiàn)配。 *5. 發(fā)表論文時引用本產(chǎn)品的寫作建議 "IHC0111R, Bioss Antibodies"。引用示例: “Rat tissue sections using Rat Histone H3 IHC Kit (IHC0111R, Bioss Antibodies) were stained for Histone H3 according to the manufacturer's instructions.” |
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| 產(chǎn)品圖片 |
Immunohistochemical analysis of paraffin embedded rat testicle tissue slide using IHC0111R (Rat Histone H3 IHC Kit).
Immunohistochemical analysis of paraffin embedded rat liver tissue slide using IHC0111R (Rat Histone H3 IHC Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
