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PDPK1 Recombinant Rabbit mAb (bsm-54037R)  
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產(chǎn)品編號 bsm-54037R
英文名稱 PDPK1 Recombinant Rabbit mAb
中文名稱 PDK1重組兔單抗
別    名 3 phosphoinositide Dependent Protein Kinase 1; hPDK 1; hPDK 1; PDK 1; PkB kinase; PkB kinase like gene 1; PDK1; PkB like 1; PDPK1_HUMAN.  3磷酸肌醇依賴性蛋白激酶1
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 2F9
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:400-800,IF=1:100-500,Flow-Cyt=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 61 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PDK1 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases.
PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.

Function:
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

Subunit:
Interacts with NPRL2.

Subcellular Location:
Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Tissue Specificity:
Appears to be expressed ubiquitously.

Post-translational modifications:
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain.

SWISS:
O15530

Gene ID:
5170

Database links:

Entrez Gene: 5170 Human

Entrez Gene: 18607 Mouse

Entrez Gene: 81745 Rat

Omim: 605213 Human

SwissProt: O15530 Human

SwissProt: Q9Z2A0 Mouse

SwissProt: O55173 Rat

Unigene: 459691 Human

Unigene: 10504 Mouse

Unigene: 10905 Rat



產(chǎn)品圖片
Sample: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate Primary: Anti-PDK1 (bsm-54037R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 61 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDK1) Monoclonal Antibody, Unconjugated (bsm-54037R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDK1) Monoclonal Antibody, Unconjugated (bsm-54037R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:NIH/3T3. Primary Antibody (green line): Rabbit Anti-PDK1 antibody (bsm-54037R) Dilution: 1:50; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1:1000. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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