| 產(chǎn)品編號 | bs-4662R-Bio |
| 英文名稱 | Rabbit Anti-phospho-TCF3 (Ser39)/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的磷酸化轉(zhuǎn)錄因子3抗體 |
| 別 名 | TCF3/E2A(phospho Ser39); p-TCF3(phospho Ser39); p-E47(phospho Ser39); p-E47(phospho Ser39); bHLHb21; Class B basic helix-loop-helix protein 21; E12; E2A; E2A immunoglobulin enhancer binding factors E12/E47; E47; Helix loop helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; ITF1; Kappa E2 binding factor; Kappa-E2-binding factor; MGC129647; MGC129648; Negative vitamin D response element binding protein; TCF-3; TCF3; TFE2; TFE2_MOUSE; transcription factor 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Chicken, Dog, ) |
| 產(chǎn)品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human TCF3 around the phosphorylation site of Ser39. |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Function: Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. Subunit: Forms a heterodimer with TWIST2. Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Isoform E12 interacts with RALGAPA1 and FIGLA. Interacts with EP300. Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Homodimer. Heterodimer. Interacts with PTF1A, TGFB1I1 and UBE2I. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with NEUROD2. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated following NGF stimulation (By similarity). Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. Database links: Entrez Gene: 6929 Human Entrez Gene: 21423 Mouse Omim: 147141 Human SwissProt: P15923 Human SwissProt: P15806 Mouse Unigene: 371282 Human Unigene: 3406 Mouse Unigene: 10290 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
