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Rabbit Anti-NCKAP1/BF488 Conjugated antibody (bs-6204R-BF488)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產品編號 bs-6204R-BF488
英文名稱 Rabbit Anti-NCKAP1/BF488 Conjugated antibody
中文名稱 BF488標記的膜相關的蛋白質HEM2抗體
別    名 NCK associated protein 1; NCK associated protein 1; Membrane associated protein HEM2; Membrane-associated protein HEM-2; NAP 1; NAP1; NAP125; NCK associated protein; Nck-associated protein 1; Nckap1; NCKP1_HUMAN; p125Nap1.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 腫瘤  細胞生物  神經(jīng)生物學  信號轉導  干細胞  細胞凋亡  轉錄調節(jié)因子  細胞膜蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Guinea Pig, G)
產品應用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 129kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human 12 NCKAP1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
NAP125, also known as NCKAP1 (NCK-associated protein 1), p125Nap1 or membrane-associated protein HEM-2, is a 1,128 amino acid single pass membrane protein that exists as two alternatively spliced isoforms and belongs to the HEM-1/HEM-2 family. While widely expressed, NAP125 is found at highest levels in heart, brain and skeletal muscle where it regulates Rac-dependent actin remodeling as part of a lamellipodial complex with WAVE2, Abi-1 and CYFIP1. NAP125 localizes to the cytoplasmic side of lamellipodium membrane and is encoded by a gene that maps to human chromosome 2q32.1 and mouse chromosome 2 C3. NAP125 expression is markedly reduced in Alzheimer disease (AD)-affected brains, suggesting a possible role in the disease.

Function:
Is part of lamellipodial complex that controls Rac-dependent actin remodeling.Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

Subcellular Location:
Cell membrane. Cell projection

Tissue Specificity:
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Similarity:
Belongs to the HEM-1/HEM-2 family.

Database links:

Entrez Gene: 3071 Human

Entrez Gene: 105855 Mouse

Entrez Gene: 315348 Rat

Omim: 141180 Human

SwissProt: P55160 Human

Unigene: 182014 Human

Unigene: 30805 Mouse

Unigene: 149002 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品圖片
Blank control: HL60. Primary Antibody (green line): Rabbit Anti-GPR65 antibody (bs-6204R-BF488) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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