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Rabbit Anti-EAAT2/FITC Conjugated antibody (bs-1751R-FITC)
訂購熱線:400-901-9800
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產品編號 bs-1751R-FITC
英文名稱 Rabbit Anti-EAAT2/FITC Conjugated antibody
中文名稱 FITC標記的膠質細胞谷氨酸運載蛋白2抗體
別    名 EAAT2; Excitatory amino acid transporter 2; Excitotoxic amino acid transporter 2; Glial high affinity glutamate transporter; GLT 1; GLT1; Glutamate aspartate transporter II; SLC1A2; Sodium dependent glutamate aspartate transporter 2; Solute carrier family 1 glial high affinity glutamate transporter member 2; Solute carrier family 1 member 2; Excitatory amino acid transporter 2; GLT-1; SLC1A2; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 免疫學  神經生物學  通道蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Rat,  (predicted: Mouse, Cow, Horse, Rabbit, )
產品應用 Flow-Cyt=3ug/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 62kDa
細胞定位 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human EAAT2
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]

Function:
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Subunit:
Homotrimer. Interacts with AJUBA.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Brain

Post-translational modifications:
Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.

Similarity:
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.

Database links:

Entrez Gene: 6506 Human

Entrez Gene: 20511 Mouse

Entrez Gene: 29482 Rat

Omim: 600300 Human

SwissProt: P43004 Human

SwissProt: P43006 Mouse

SwissProt: P31596 Rat

Unigene: 502338 Human

Unigene: 267547 Mouse

Unigene: 371582 Mouse

Unigene: 10240 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品圖片
Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-EAAT2 antibody (bs-1751R-FITC) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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