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Rabbit Anti-GLMN/BF647 Conjugated antibody (bs-7030R-BF647)
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說(shuō) 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) bs-7030R-BF647
英文名稱 Rabbit Anti-GLMN/BF647 Conjugated antibody
中文名稱 BF647標(biāo)記的FK506結(jié)合蛋白相關(guān)蛋白抗體(他克莫司相關(guān)蛋白)
別    名 FAP; FAP48; FAP68; FK506 binding protein associated protein; FKBP associated protein; FKBPAP; GLML; GLMN; Glomulin; GVM; VMGLOM.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說(shuō) 明 書 100ul  
研究領(lǐng)域 干細(xì)胞  血管內(nèi)皮細(xì)胞  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,  (predicted: Mouse, Rat, Dog, Pig, Horse, )
產(chǎn)品應(yīng)用 Flow-Cyt=3ug/Test IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 68 kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLMN/FK506 binding protein associated protein
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
GLMN is a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Alternatively spliced variants that encode different protein isoforms have been described but the full length nature of only one has been determined. GLMN may represent a naturally occurring ligand of the immunophilins FKBP59 and FKBP12 and may function as an membrane anchoring protein. Isoform 1 may stimulate the p70S6K pathway. Isoform 2 may inhibit cell proliferation and increase IL2 production.

Function:
Essential for normal development of the vasculature. May represent a naturally occurring ligand of the immunophilins FKBP59 and FKBP12. May function as an membrane anchoring protein. Isoform 1 may stimulate the p70S6K pathway. Isoform 2 may inhibit cell proliferation and increase IL2 production.

Subunit:
Monomer. Isoform 1 interacts with notphosphorylated MET and is released upon receptor phosphorylation. Isoform 2 interacts with FKBP59 and FKBP12. Isoform 1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylated on tyrosine residues.

DISEASE:
Defects in GLMN are the cause of glomuvenous malformations (GVMs) [MIM:138000]. GVMs are characterized by the presence of smooth-muscle-like glomus cells in the media surrounding distended vascular lumens.

Database links:

Entrez Gene: 504211 Cow

Entrez Gene: 11146 Human

Entrez Gene: 170823 Mouse

GenBank: NP_444504.1 Human

Omim: 601749 Human

SwissProt: Q92990 Human

SwissProt: Q8BZM1 Mouse

Unigene: 49105 Human

Unigene: 41417 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片
Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-GLMN antibody (bs-7030R-BF647) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-AF647 . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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