亚洲制服欧美另类-午夜激情av电影-日本高清中文字幕一区二区三区-中国欧美日韩一区二区三区-欧洲亚洲日本韩国-成人欧美激情一区二区-亚洲偷偷自拍高清

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質(zhì)量反饋  關于我們  聯(lián)系我們
日韩精品亚洲一区二区三区,欧美亚洲精品中文字幕乱码免费高清
首頁 > 產(chǎn)品中心 > 標記一抗 > 產(chǎn)品信息
Rabbit Anti-RNA polymerase II/PE Conjugated antibody (bs-6972R-PE)
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-6972R-PE
英文名稱 Rabbit Anti-RNA polymerase II/PE Conjugated antibody
中文名稱 PE標記的RNA聚合酶II抗體
別    名 POLR2A; POLR2; DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; RPB1_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  免疫學  信號轉導  轉錄調(diào)節(jié)因子  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, )
產(chǎn)品應用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 217kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Function:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

Subunit:
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C (By similarity). Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5.

Subcellular Location:
Nucleus.

Post-translational modifications:
Dephosphorylated by the protein phosphatase CTDSP1. [PTM] Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). [PTM] Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs.

Similarity:
Belongs to the RNA polymerase beta' chain family.

Database links:
 

UniProtKB/Swiss-Prot: P24928.2



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
版權所有 2004-2026 www.a6308.cn 北京博奧森生物技術有限公司
通過國際質(zhì)量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫(yī)療器械-質(zhì)量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網(wǎng)安備110107000727號
国产亚洲色婷婷久久99精品 | 一本一本久久a久久精品66 | 亚洲乱码日产精品BD在线看 | 又色又爽又黄又粗暴小说 | 在线免费观看三级片国产 | 大桥未久加勒比女热大陆在线 | 日韩特黄特色大片免费视频 | 国产精品亚洲第一区在线 | 九九在线中文字幕无码 | ww久久综合久中文字幕老太婆 | 九九热这里只有国产精品 | 欧美三级片a片在线观看 | 日韩在线视频观看免费网站 | 亚洲人人夜夜澡人人爽 | 天堂tv亚洲tv日本tv不卡 | 小明精品国产一区二区三区 | 欧美透明丰满内衣内裤 | √天堂中文www官网在线 | 99久在线国内在线播放免费观看 | 亚洲春色Av无码专区 | www免费视频在线观看播放 | 免费欧美黄色网址 | 久久久久久精品免费自慰 | 欧美极品欧美精品欧美视频 | 国产在线观看免费一级 | 国产麻豆精品久久一二三 | 色欲av无码一区二区人妻精油 | 手机在线观看免费AV网址 | 九九线精品视频在线观看视频 | 少妇被躁爽到高潮无码麻豆AV | 好吊妞www在线观看免费 | 爱啪网亚洲第一福利网站 | 黑人巨大精品欧美一区二区 | 内射无码AV-区二区三区 | 精品无码国产自产野外拍在线 | 日本特级婬片a片免费手机版 | 国产精品免费视频能看 | 精品欧洲视频人人视频网站 | 日本韩国亚洲综合日韩欧美国产 | 国产又猛又黄又爽 | 成人看片黄a在线看 |